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Abstract

In Vitro Susceptibility of Commonly Used Antibiotics against Vibrio parahaemolyticus, Isolated from Finfishes, Chennai, India

Purpose: The main aim of this study is to determine the antibiotic profile of Vibrio parahaemolyticus isolated from fin-fishes collected from three major fish landing sites in Chennai, India.

Methods: Antibiotic profiles of Gram-negative halophilic bacterium Vibrio parahaemolyticus isolated from skin surfaces of a total of 112 fin-fish samples, consisting of 30 Red snapper (Lutjanus campechanus), 40 Indian sardine (Sardinella longiceps) and 42 Rohu (Labeo rohita) fishes were determined by disc diffusion method, using commonly used antibiotic discs, doxycycline, ofloxacin, cefazolin, clindamycin, gentamycin and chloramphenicol. Kanagawa reaction was further performed, to detect the pathogenicity of the identified Vibrio parahaemolyticus strains.

Results/Findings: Thirty two samples were found to be positive. Among 32 isolates, 5 were found to be positive for kanagawa reaction. The isolated Vibrio parahaemolyticus strains showed significant in vitro susceptibility to doxycycline and ofloxacin.


Author(s):

Muralidharan Velappan and Deecaraman Munusamy



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